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《医学前沿(英文)》 2022年 第16卷 第6期 页码 919-931 doi: 10.1007/s11684-021-0918-6
关键词: preeclampsia recombinant protein diannexin microparticle NLRP3 inflammasome phosphatidylserin
Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT
《化学科学与工程前沿(英文)》 2012年 第6卷 第2期 页码 216-223 doi: 10.1007/s11705-012-1289-y
关键词: recombinant protein polyclonal antibody label-free biosensor impedance white spot syndrome virus (WSSV)
High-expression of recombinant human concensus interferon-α by Pichia pastoris
HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin
《化学科学与工程前沿(英文)》 2007年 第1卷 第4期 页码 399-403 doi: 10.1007/s11705-007-0073-x
Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus
Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE
《医学前沿(英文)》 2009年 第3卷 第1期 页码 67-71 doi: 10.1007/s11684-009-0010-0
关键词: divalent metal transporter 1 recombinant adenovirus homologous recombination iron
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 382-389 doi: 10.15302/J-FASE-2018211
The mammary gland provides a novel method for producing recombinant proteins in milk of transgenic animals. A key component in the technology is the construction of an efficient milk expression vector. Here, we established a simple method to construct a milk expression vector, by a combination of homologous recombination and digestion-ligation. Our methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BAC) vectors. The BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombination to produce a universal expression vector, and the human lysozyme gene (hLZ) was then inserted into the vector by a digestion-ligation method. The final vector containing the 8.5 kb mWAP 5′ promoter, 4.8 kb hLZ genomic DNA, and 8.0 kb mWAP 3′ genomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant human lysozyme (rhLZ) in milk. The highest expression level of rhLZ was 0.45 g·L−1, and rhLZ exhibited the same antibacterial activity as native hLZ. Our results have provided a simple approach to construct a universal milk expression vector, and demonstrated that the resulting vector regulates the expression of hLZ in milk.
关键词: BAC recombinant methods gene expression human lysozyme transgenic mice milk expression vector
Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU
《医学前沿(英文)》 2009年 第3卷 第2期 页码 171-176 doi: 10.1007/s11684-009-0027-4
关键词: recombinant human growth hormone injection growth hormone deficiency
Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng
《医学前沿(英文)》 2018年 第12卷 第5期 页码 580-585 doi: 10.1007/s11684-017-0585-9
This study aimed to evaluate the effects of thyroid hormone supplementation on growth rate of children with idiopathic short stature (ISS) and low-normal serum free thyroxine FT4 who were receiving growth hormone therapy. We selected 64 prepubertal children with FT4 levels in the lowest third of the normal range as the lower FT4 group, and these children were divided randomly into two subgroups: L-thyroxine (L-T4)-treated subgroup was treated with L-T4 (0.5–3.0 g/(kg·d)) from the beginning of the study, and the non-L-T4-treated subgroup received placebo. We also selected 39 ISS children with FT4 in the upper two-thirds of the normal range as the higher FT4 group. During the first year, the lower FT4 group featured lower FT3, FT4, thyroid stimulating hormone (TSH), and insulin-like growth factor-I standard deviation score (IGF-I SDS) and significantly lower height velocity (HV) compared with the higher FT4 group. However, in the lower FT4 group, the L-T4-treated subgroup presented higher FT4, FT3, TSH, and IGF-I SDS concentrations and significantly higher HV compared with children in the non-L-T4-treated subgroup. In children with ISS, the negative effect of thyroid hormone deficiency on growth rate should be considered when FT4 level lies in the low-normal range prior to recombinant human growth hormone treatment.
关键词: therapeutic idiopathic short-stature children free T4 the first year recombinant human growth hormone
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
《农业科学与工程前沿(英文)》 2014年 第1卷 第3期 页码 179-184 doi: 10.15302/J-FASE-2014020
关键词: recombinant human butyrylcholinesterase (rhBCHE) human lactoferrin bacterial artificial chromosome (hLF BAC) transgenic mice milk
Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction
Hong LI,Ziding ZHANG
《农业科学与工程前沿(英文)》 2016年 第3卷 第2期 页码 102-112 doi: 10.15302/J-FASE-2016100
关键词: plant–pathogen interactions systems biology omics plant immunity protein–protein interaction network
High-level expression of recombinant IgG1 by CHO K1 platform
Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu
《化学科学与工程前沿(英文)》 2015年 第9卷 第3期 页码 376-380 doi: 10.1007/s11705-015-1531-5
关键词: Chinese hamster ovary (CHO) monoclonal antibody IgG1 amplification cell line development
Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus
Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO
《医学前沿(英文)》 2009年 第3卷 第2期 页码 158-163 doi: 10.1007/s11684-009-0042-5
关键词: tetracycline regulatory system adeno-associated virus basic fibroblast growth factor gene regulation
The role of protein kinase C epsilon in neural signal transduction and neurogenic diseases
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 70-76 doi: 10.1007/s11684-011-0119-9
Protein kinase C epsilon (PKC ?) is one of major isoforms in novel PKC family. Although it has been extensively characterized in the past decade, the role of PKC ? in neuron is still not well understood. Advances in molecular biology have now removed significant barriers to the direct investigation of PKC ? functions in vivo, and PKC ? has been increasingly implicated in the neural biological functions and associated neurogenic diseases. Recent studies have provided important insights into the influence of PKC ? on cortical processing at both the single cell level and network level. These studies provide compelling evidence that PKC ? could regulate distinct aspects of neural signal transduction and suggest that the coordinated actions of a number of molecular signals contribute to the specification and differentiation of PKC ? signal pathway in the developing brain.
关键词: protein kinase C ? signal transduction neurogenic disease
FAN Xiaodong, HAN Ruifa
《医学前沿(英文)》 2007年 第1卷 第4期 页码 377-380 doi: 10.1007/s11684-007-0073-8
Wheat gluten protein and its impacts on wheat processing quality
Wujun MA, Zitong YU, Maoyun SHE, Yun ZHAO, Shahidul ISLAM
《农业科学与工程前沿(英文)》 2019年 第6卷 第3期 页码 279-287 doi: 10.15302/J-FASE-2019267
Before the advent of the wheat genomic era, a wide range of studies were conducted to understand the chemistry and functions of the wheat storage proteins, which are the major determinants of wheat flour the suitability of wheat flour for various end products, such as bread, noodles and cakes. Wheat grain protein is divided into gluten and non-gluten fractions and the wheat processing quality mainly depends on the gluten fractions. Gluten provides the unique extensibility and elasticity of dough that are essential for various wheat end products. Disulfide bonds are formed between cysteine residues, which is the chemical bases for the physical properties of dough. Based on the SDS-extractability, grain protein is divided into SDS-unextractable polymeric protein (UPP) and SDS-extractable polymeric protein. The percentage of UPP is positively related to the formation of disulfide bonds in the dough matrix. In the wheat genomic era, new glutenins with long repetitive central domains that contain a high number of consensus hexapeptide and nonapeptide motifs as well as high content of cysteine and glutamine residues should be targeted.
关键词: wheat gluten consensus motifs disulfide bonds SDS-unextractable polymeric protein glutenins gliadins processing quality storage protein
《化学科学与工程前沿(英文)》 2023年 第17卷 第8期 页码 1131-1139 doi: 10.1007/s11705-023-2321-0
关键词: biomass riboflavin sodium phosphate silk protein multifunctional modification flame retardant
标题 作者 时间 类型 操作
Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing
期刊论文
Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv
Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT
期刊论文
High-expression of recombinant human concensus interferon-α by Pichia pastoris
HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin
期刊论文
Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus
Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE
期刊论文
Construction of a universal recombinant expression vector that regulates the expression of human lysozyme
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
期刊论文
Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency
Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU
期刊论文
supplementation of thyroid hormone on treatment of idiopathic short-stature children during therapy with recombinant
Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng
期刊论文
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
期刊论文
Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction
Hong LI,Ziding ZHANG
期刊论文
High-level expression of recombinant IgG1 by CHO K1 platform
Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu
期刊论文
Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus
Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO
期刊论文
The role of protein kinase C epsilon in neural signal transduction and neurogenic diseases
null
期刊论文
Study of recombinant human IFN-α-2b bacilli Calmette–Guerin activated killer cells and against bladder
FAN Xiaodong, HAN Ruifa
期刊论文
Wheat gluten protein and its impacts on wheat processing quality
Wujun MA, Zitong YU, Maoyun SHE, Yun ZHAO, Shahidul ISLAM
期刊论文